Fig. 8

miRNA regulation models during Callus formation and long-term subculture. The regulation model of miRNA on transcriptional reprogramming in Callus cells. All the pathways in Figure were significantly differentially expressed, and the thickness of the arrows indicated the regulation strength by miRNAs. Some pathways also significantly changed but they were not regulated by miRNA, such as isoflavonoid biosynthesis. The pathways in green indicated most DEGs of these pathways were downregulated, red indicated most DEGs were upregulated, black means the upregulated genes of these pathways were as much as the downregulated ones. Most pathways of secondary metabolism were barely regulated by miRNAs directly, probably the regulation model was a “miRNA-TF-enzyme genes” mode