Fig. 5

Genotyping results of 3RS-specific Kompetitive allele-specific PCR (KASP) Marker SWK28002 (a) and 6RL-specific KASP Marker SWK26615 (b). Orange rotund shapes that represent homozygous rye-derived specific SNP ‘Allele1/Allele1’ indicate 12 rye cultivars including KingII, German White, Imperial, Jingzhouheimai, Baili, Guyuan, CIse 1, CIse 12, CIse14, CIse17, CIse53 and CIse54; blue square shapes that represent homozygous wheat-derived specific SNP ‘Allele2/Allele2’ indicate two sets of disomic addition lines of ‘Holdfast-KingII’ and ‘Chinese Spring-Imperial’ without 3R/6R disomic addition lines, a set of ditelosomic addition lines of ‘Holdfast-KingII’ without 3RS/6RL ditelosomic addition line, and 11 wheat materials including Holdfast, Chinese Spring, Shixin633, Shixin733, Shixin828, Gao8901, Jishi02–1, Shimai15, Kenong199, Heng5471 and Jimai22; and green triangle shapes that represent heterozygous rye- and wheat- derived SNP ‘Allele1/Allele2’ indicate 3R/6R disomic addition line and 3RS/6RL ditelosomic addition line of ‘Holdfast-KingII’ and 3R/6R disomic addition line of ‘Chinese Spring-Imperial’. Black diamond shapes indicate no template control