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Fig. 2 | BMC Genomics

Fig. 2

From: Phylogenetic relationship between Australian Fusarium oxysporum isolates and resolving the species complex using the multispecies coalescent model

Fig. 2

Phylogenetic analysis of whole genome of Fusarium oxysporum isolates used in the current study and eight reference isolates (NRRL25433, NRRL54005, NRRL54008, NRRL26406, Forc016, Fol4287, Foc013 and Foc011) included from GenBank using Roary: Pan Genome pipeline. Six thousand eight hundred genes were found to be conserved across all the isolates including the outgroup, Fusarium proliferatum (ITEM2341 and NRRL62905). The protein sequences for these genes were aligned using Multiple Alignment FAST Fourier Transform (MAFFT) [46] and then clustered into orthologous groups. A Maximum Likelihood tree was generated using FastTree [47] with the General Time Reversal (GTR) substitution model. FastTree used the Shimodaira-Hasegawa (SH) test for three alternate topologies for every split and each split was sampled 1000 times. The five phylogenetic clades and sub-clades identified within the FOSC are marked

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