Fig. 3

B. oleae polytene chromosomes mapping of molecular markers Y chromosome assembly. a Plot showing Y chromosome scaffolds/contigs identified in 2 different assemblies (Supplementary Table S4). The Chromosome Quotient (CQ) method [49] was used to identify Y chromosome scaffolds. The scaffolds/contigs are ordered from longest at the bottom to shortest at the top. For each assembly the total scaffolds/contigs are shown in left bars while the PCR validated scaffolds/contigs are the right bars. The approximate location of the PCR primer on the scaffold/contig is shown in pink. b Schematic representation of B. oleae polytene chromosomes including all mapped markers (tags) and the scaffolds assigned to chromosomes. Previously and currently mapped markers are indicated with black and red letters, respectively, above chromosomes. Colored horizontal bars above chromosomes indicate scaffolds/contigs in the GCA_001188975.4 assembly that were localized to chromosomes using mapped markers. More than one tags on a specific scaffold is informative of its physical orientation. m## corresponds to microsatellite markers number ##; c## corresponds to EST marker number ## [26, 50]; newly mapped genes in the current study are presented in full names or abbreviations (Supplementary Table S6); “*” indicates the tags that were not found on the anchored contig or gave ambiguous alignment results. The centromere is shown as a filled circle. (see Supplementary Table S7 for detailed information)