Fig. 2

Cell-type populations at early stages of cortical development. a Apical radial glial cells (RGCs) populate the ventricular zone and prolong one process apically to the ventricular surface and another one to the basal lamina, which serves as a scaffold for neuronal migration. RGCs also proliferate and differentiate to give rise to another RGC, a basal intermediate progenitor (indirect neurogenesis), or a neuron (direct neurogenesis) [28]. Intermediate progenitor cells (IPCs) are basal progenitors lacking of apical-basal cell polarity. IPCs migrate to the subventricular zone and, after a couple of self-renewal divisions, differentiate to give rise to two neurons [28]. b The tSNE plot shows twelve clusters detected analyzing a total of 762 cells. c The violin plots show expression of two markers (PAX6, EOMES) across the different clusters,distinguishing between RGCs and IPCs. d The miniature tSNE plots show the distribution across the clusters of a selection of genes discussed in the main text.IPC: Intermediate progenitor cells; NascentN: Nascent neurons; ExN: Excitatory neurons; Astro:Astrocytes; RGC: Radial glial cells; InN: Interneurons; Oligodendrocyte progenitor cells: OPC;Oligo: Oligodendrocytes; Cl12: Cluster 12 (unidentified cells)