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Table 4 Heterozygous mutations in genes involved in DNA repair

From: Genomic analysis of a riboflavin-overproducing Ashbya gossypii mutant isolated by disparity mutagenesis

ChromosomePositionWT seq.MT seqQualityMutationGeneProductDNA changesProtein changesRead numberMT seq ratio
WT seq.MT seq.
I177,825GT533.19missenseAGOS_AAL093CDNA mismatch repair protein (AgMLH3)c.1516C > Ap.L506M22170.436
IV997,942TG729.19missenseAGOS_ADR168CDNA mismatch repair protein (AgMSH3)c.2937A > Cp.K979N39230.371
IV998,607GA982.19nonsenseAGOS_ADR168CDNA mismatch repair protein (AgMSH3)c.2272C > Tp.Q758*22290.569
IV1,446,658GT747.19missenseAGOS_ADR411WCheckpoint protein (AgRAD17)c.358G > Tp.D120Y32240.429
V486,710GA711.19missenseAGOS_AEL075WDNA polymerase delta subunit 3 (AgPOL32)c.490G > Ap.A164T18220.550
V1,239,357CA411.19missenseAGOS_AER327CUracil-DNA glycosylase (AgUNG1)c.757G > Tp.A253S27160.372
V1,445,972GA869.19missenseAGOS_AER421WDNA mismatch repair protein (AgPMS1)c.1762G > Ap.A588T23280.549
VI65,368CT587.19missenseAGOS_AFL199CDNA mismatch repair protein (AgMLH1)c.320G > Ap.C107Y20200.500
VI677,447GA967.19nonsenseAGOS_AFR133Csingle-stranded DNA endonuclease (AgRAD2)c.2143C > Tp.Q715*25300.545
VI677,525CA743.19missenseAGOS_AFR133Csingle-stranded DNA endonuclease (AgRAD2)c.2065G > Tp.D689Y27280.509
VI834,113AG889.19missenseAGOS_AFR220WDNA helicase/Ubiquitin ligase (AgRAD5)c.2419A > Gp.S807G18250.581
VI834,860GT678.19nonsenseAGOS_AFR220WDNA helicase/Ubiquitin ligase (AgRAD5)c.3166G > Tp.E1056*29220.431
VI848,262AG828.19missenseAGOS_AFR226CDNA mismatch repair protein (AgMLH2)c.1882 T > Cp.F628L26310.544
VI1,528,970CT1142.19nonsenseAGOS_AFR603CDNA mismatch repair protein (AgMSH2)c.2711G > Ap.W904*18360.667
VI1,529,553GA1151.19missenseAGOS_AFR603CDNA mismatch repair protein (AgMSH2)c.2128C > Tp.P710S12330.733
VII1,278,725TG786.19missenseAGOS_AGR116WDNA mismatch repair protein (AgMSH6)c.1005 T > Gp.N335K24250.510
VII1,368,167CT788.19missenseAGOS_AGR162CDNA repair protein (AgRAD4)c.1214G > Ap.R405Q17230.575
  1. *Translation stops here