Fig. 4
From: Comparative genomics of whole-cell pertussis vaccine strains from India
Identification of single nucleotide polymorphism associated with antibiotic resistance using multiple genome alignment. Multiple sequence alignment generated using MEGA 9, with sequence of the 23S rRNA gene of Bordetella pertussis as reference sequence, showing sequence similarity of clinical strains BPD1 and BPD2 from India with 23S rRNA gene of Bordetella pertussis of Tohama-I and Chinese vaccine strain at position A2047G based on old Tohama-I 23S rRNA gene of Tohama-I. Absence of nucleotide variation observed at position describing mutation associated with antibiotic resistance (binding site of erythromycin)