Fig. 6

Relative expression of flower induction genes. For the NL and L0 samples, the relative expression of genes and transcription factors involved in the flowering process was determined via quantitative RT-PCR. Expression was normalized to that of UBQ. The transcript levels from the NL sample were set as 1. The blue columns represent the expression levels determined by qRT-PCR (left axis), whereas the lines represent the gene expression levels determined by RNA-Seq (right axis). The transcript abundances of the genes encoding a cycling DoF factor 1 protein, b zinc finger protein CONSTANS-LIKE 2, c HEADING DATE 3A-like, and d response regulator-like APRR5 were determined and compared across the time course of light-induced treatment. Data represent the mean ± SD for three replicates (n = 3). Different lowercase letters above the columns indicate a significant difference at p ≤ 0.05 between the columns according to t tests performed using the SPSS statistical software. Data in columns with the same letters showed no significant difference (p > 0.05)