Fig. 6

DSM 20052 CRISPR transcription. a Loci comparison of the putative Type II-A CRISPR-Cas loci in DSM 20052 (top) and LT906621 (bottom). cas genes are annotated. The repeat spacer arrays are visualized with the standard diamond/rectangle scheme. mRNA data for DSM 20052’s loci is overlaid. smRNA-seq expression profiles for the (b) repeat spacer array, (c) crRNA (yellow), (d), ldrRNA (green), and (e) tracrRNA (blue), the sequencing coverage is on the y-axis. In (b), the right y-axis shows the log transformed coverage for the boxplots. The leader is highlighted in green. In (c), the spacer (rectangle) and repeat (diamond) boundaries are labelled. In (d), the leader and repeat (diamond) boundaries are labelled. In (e), the predicted boundary is in gray and the actual is labelled. In (f) WebLogo of the proposed PAM sequence for the system. g Predicted structure of the tracrRNA with the lower stem (green), bulge (yellow), nexus (blue), and hairpin (red) highlighted