Fig. 4

PCR analysis of the γ-CA gene from F. catus and β-CA gene from M. musculus. Samples from two animals of both species were included in the analysis, and primer pairs P1, P3, P5, and P8 were selected based on preliminary experiments. a shows the results from the first round of PCR. The bands nearest to the estimated correct size (red arrows) are marked with red circles (1–9). These bands were isolated, and the purified DNAs were used as templates for the second round of PCR. The results are shown in b. The amplified products from samples 3, 4, 8, and 9 were subsequently subjected to DNA sequencing