Fig. 4

Examples of PQS motifs and their variation presented as LOGO sequences a. PQS with the highest G4Hunter score (1.4), b. PQS with the lowest G4Hunter score (− 1.2; a negative score indicates that the G-rich motif is located in negative gRNA), c. PQS with the most variable sequence (G4Hunter score 1.2) from NP segment, d. PQS with conserved GGGG-tracks (1.1) and e. PQS conserved sequence (− 1.2,). Perfectly conserved nucleotides are represented by full size letters. All sequence logos are shown in SM_04