Fig. 3
From: Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing
Discrepancy in the Relationship Between Noise and RIN due to additional Freeze-Thaw. Examining the relationship between noise, calculated by Euclidean distance, RMSE, Pearson, and Spearman correlation, for samples that underwent either one (blue) or two (red) freeze-thaw cycles. a Scatter plots comparing noise (y-axis) to RIN (x-axis). The solid lines show a linear regression fit and the shaded regions show the 95% confidence interval for this fit. b The expected change in noise due to a one-point increase in RIN (ΔN, y-axis) estimated by linear regressions. Significant estimates (p ≤ 0.05) are marked by a circle and insignificant estimates are marked by a cross