Fig. 4

Phylogenetic relationship among membrane-associated NAC TFs from pearl millet, Setaria italica, Brachypodium distachyon and maize. Multiple sequence alignment of all putative membrane-associated NAC TFs in pearl millet (proteins with “PgNAC” in their names), Setaria italica (proteins with “Seita”), Brachypodium distachyon (proteins with “Bradi”) and maize (proteins with “GRMZM”) was conducted using ClustalW. MEGA 7.0 was used to create phylogenetic trees using the neighbor-joining method with 1000 bootstrap replicates and the p distance method