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Table 1 The organism-wide ΔRSCU values determined using genes with the top 5% expression level (when averaged across all nine tissues) and lowest 5% expression level (**P < 0.001), the predicted tRNA numbers, and codon statuses.

From: Adaptation of codon and amino acid use for translational functions in highly expressed cricket genes

Amino acid Codon
(DNA)
Standard anticodon ΔRSCU Pa No. tRNAs Optimal and non-optimal status Wobble
anticodon
(optimal)b
Ala GCT AGC + 0.871 ** 35 Opt-codon↑tRNAs  
Ala GCC GGC −0.344 ** 0  
Ala GCA UGC + 0.518 ** 18  
Ala GCG CGC −1.039 ** 22 Nonopt-codon↑tRNAs  
Arg CGT ACG + 0.463 ** 40  
Arg CGC GCG −1.053 ** 0 Nonopt-codon↓tRNAs  
Arg CGA UCG + 0.185 ** 39  
Arg CGG CCG −0.548 ** 2  
Arg AGA UCU + 0.881 ** 18 Opt-codon↑tRNAs  
Arg AGG CCU + 0.047   26  
Asn AAT AUU + 0.416 ** 0 Opt-codonwobble GUU
Asn AAC GUU −0.244 ** 37 Nonopt-codon↑tRNAs  
Asp GAT AUC + 0.520 ** 0 Opt-codonwobble GUC
Asp GAC GUC −0.482 ** 31 Nonopt-codon↑tRNAs  
Cys TGT ACA + 0.368 ** 0 Opt-codonwobble GCA
Cys TGC GCA −0.365 ** 38 Nonopt-codon↑tRNAs  
Gln CAA UUG + 0.254 ** 39 Opt-codon↑tRNAs  
Gln CAG CUG −0.218 ** 37 Nonopt-codon↑tRNAs  
Glu GAA UUC + 0.496 ** 31 Opt-codon↑tRNAs  
Glu GAG CUC −0.480 ** 18 Nonopt-codon↑tRNAs  
Gly GGT ACC + 0.610 ** 0 Opt-codonwobble GCC
Gly GGC GCC −0.709 ** 41 Nonopt-codon↑tRNAs  
Gly GGA UCC + 0.483 ** 19  
Gly GGG CCC −0.383 ** 11  
His CAT AUG + 0.511 ** 0 Opt-codonwobble GUG
His CAC GUG −0.452 ** 37 Nonopt-codon↑tRNAs  
Ile ATT AAU + 0.603 ** 22 Opt-codon↑tRNAs  
Ile ATC GAU −0.452 ** 0 Nonopt-codon↓tRNAs  
Ile ATA UAU + 0.045   19  
Leu TTA UAA + 0.537 ** 28 Opt-codon↑tRNAs  
Leu TTG CAA + 0.383 ** 16  
Leu CTT AAG + 0.409 ** 39  
Leu CTC GAG −0.629 ** 0  
Leu CTA UAG + 0.007   28  
Leu CTG CAG −0.692 ** 30 Nonopt-codon↑tRNAs  
Lys AAA UUU + 0.263 ** 20 Opt-codon↑tRNAs  
Lys AAG CUU −0.160 ** 50 Nonopt-codon↑tRNAs  
Phe TTT AAA + 0.407 ** 0 Opt-codonwobble GAA
Phe TTC GAA −0.265 ** 48 Nonopt-codon↑tRNAs  
Pro CCT AGG + 0.749 ** 36 Opt-codon↑tRNAs  
Pro CCC GGG −0.359 ** 0  
Pro CCA UGG + 0.483 ** 31  
Pro CCG CGG −0.843 ** 36 Nonopt-codon↑tRNAs  
Ser TCT AGA + 0.731 ** 36 Opt-codon↑tRNAs  
Ser TCC GGA −0.208 ** 0  
Ser TCA UGA + 0.493 ** 21  
Ser TCG CGA −0.723 ** 15 Nonopt-codon↑tRNAs  
Ser AGT ACU + 0.325 ** 0  
Ser AGC GCU −0.619 ** 60  
Thr ACT AGU + 0.644 ** 35 Opt-codon↑tRNAs  
Thr ACC GGU −0.223 ** 0  
Thr ACA UGU + 0.493 ** 37  
Thr ACG CGU −0.873 ** 31 Nonopt-codon↑tRNAs  
Tyr TAT AUA + 0.430 ** 0 Opt-codonwobble GUA
Tyr TAC GUA −0.186 ** 43 Nonopt-codon↑tRNAs  
Val GTT AAC + 0.600 ** 26 Opt-codon↑tRNAs  
Val GTC GAC −0.394 ** 0  
Val GTA UAC + 0.314 ** 30  
Val GTG CAC −0.484 ** 40 Nonopt-codon↑tRNAs  
Amino acids with one codon     
Met ATG CAU    43  
Trp TGG CCA    32  
Total tRNAs      1391   
  1. The number of predicted tRNAs are shown [69]. The primary optimal codon per amino acid and its ΔRSCU value are in bold and underlined. The status of an optimal codon that has a relatively high number of tRNAs (≥18) and those with no tRNAs, and thus obligately requiring the use of wobble tRNAs, are shown, as well as the putative wobble anticodon. The status of primary non-optimal codons that have matching tRNA gene numbers substantially in excess of 0 (≥15) and those with few/no tRNAs are indicated. The status categories are further described in the main text. Codons not having primary optimal or non-optimal status are indicated by “--“.a, α = 0.05, all "**" contrasts had P < 0.001, including after Bonferonni correction. b Standard wobble codons provided; see also inosine modified anticodons for codons with no exact matching tRNAs [70, 71].
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