Fig. 7
From: Borrelia burgdorferi infection modifies protein content in saliva of Ixodes scapularis nymphs
Tick saliva proteins eliciting immunity against Ixodes scapularis nymph feeding are apparently highly expressed in Borrelia burgdorferi infected ticks than uninfected. Total tick protein extracts (500 ng per well) of uninfected (blue) and B. burgdorferi infected (red) I. scapularis nymphs that fed on rabbits for 24, 48, and 72 h were subjected to standard ELISA using purified IgG (100 μg/mL) of rabbits that were exposed once (Ab97 and 98) or twice (Ab 24, 25, 26, 27, 48, 49, 50 and 51) to uninfected and infected ticks. Y-axis = A450nm, X-axis shows different rabbit antibodies. P/immune = pre-immune, UINF-Ab98, 25, 27, 50, and 51 = rabbit immune sera to tick saliva proteins of uninfected nymphs. BbINF-Ab97, 24, 26, 48, and 49 = rabbit immune sera to tick saliva proteins of B. burgdorferi infected nymphs. A, B, and C = ticks that fed for 24, 48, and 72 h respectively