Fig. 5

Hepatic transcript expression changes (qPCR-Fluidigm Biomark) for 23 target genes. Relative expression values [i.e., relative quantity (RQ)] per individual gene are plotted for Warm & Normoxic (WN: 20 °C, 100% air saturation) and Warm & Hypoxic (WH: 20 °C, ~ 70% air sat.) fish as compared to fish of the Control group (CT: 12 °C, 100% air sat.) (n = 8 per treatment, N = 24 total). The horizontal line within the box plots indicates the median value, the top and bottom limits of the bar indicate the 25th and 75th quartiles, the vertical lines indicate the maximum and minimum values, and the gray dots indicate outliers. Significant differences between groups are indicated by different capital letters (lsmeans post-hoc test, p < 0.05) and non-significant differences are shown by a p-value (p > 0.05) (see Table 3). The plots are sorted according to six functional themes. Heat Shock Response: a serpinh1, b hsp90aa1, c hsp70; Cellular and Oxidative Stress Response: d hcn1, e cirbp, f calm, g cyp1a1, h egln2, i prdx6, j rraga, k ucp2, l hif1α and m gstt1; Transcription: n dnmt1; Metabolism: o pdk3; Apoptosis: p casp8, q jund, r jak2 and s ctsh; and Immune Response: t apod, u c1ql2, v epx and w nckap1l