Fig. 6

smFiSH analysis of sex-biased and TCPOBOP-responsive lncRNAs. a Schematic describing ACD’s RNAScope technology. ZZ probes are hybridized to the gene of interest, then pre-amplifier and amplifiers build in a tree-and-branch manner to amplify the probe/label signal up to 8000-fold, enabling visualization of single RNA molecules. Visualization of expression for: b lnc7423, a male-biased lncRNA; c lnc14770, female-biased lncRNA, using ZZ probes designed to a small region of each target lncRNA; and d lnc5998, a TCPOBOP-inducible lncRNA, and the nearby PCG, Cyp2b10, using ZZ probes designed to a small region of lnc5998, and across the exonic structure of Cyp2b10 for vehicle control and 51 h-TCPOBOP-exposed female mouse liver slices. Shown are expression levels (FPKM values, from nuclear RNA-seq, for each gene). See Table S1D for lncRNA genomic coordinates and annotations, Figure S6 for quantitative analysis of smFISH data for all four genes in both sexes, and Table S7 for FPKM values for each gene in each subcellular fraction