Skip to main content
Fig. 3 | BMC Genomics

Fig. 3

From: Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses

Fig. 3

The induced expression level of miR827 (root ID: 114, shoot ID: 2073) correlates with downregulation of its target SPX-MFS1 in barley. a The absolute gene expression quantification of identified mature hvu-miR827 and its predicted target gene SPX-MFS1 using ddPCR. The bars represent copy numbers normalized to 1000 copies of the ARF1 reference gene; * p-value < 0.05, calculated using two-tailed Student’s t-tests for three biological and two technical replicates. Error bars = SD. b Detection of hvu-miR827 expression pattern in barley samples used in this study for NGS analysis. Specific probes for hvu-miR827 mature sequence and U6 reference gene were used for Northern hybridization performed on a single membrane. The number represents hvu-miR827 band intensity compared to U6 snRNA. The blots were cropped and original, full-length blots are presented in Additional files 32 and 33

Back to article page
\