Fig. 4

(a) 4-way Venn diagram analysis shows numbers of significant adrenal differentially expressed genes (DEGs) and their overlapping categorization using multiple comparisons with fold-changes (FC≥± 1.4, FDR < 0.05) for Set 1: male KK/HlJ versus male C57BL/6 J (KK-M vs. C57-M, violet); Set 2: male KK/HlJ versus female KK/HlJ (KK-M vs. KK-F, yellow), Set 3: female KK/HlJ versus female C57BL/6 J (KK-F vs. C57-F, green), and Set 4: male C57BL/6 J versus female C57BL/6 J (C57-M vs. C57-F, pink). (b) Heatmap with hierarchical clustering of differentially expressed adrenal genes (FDR < 0.05) between the 4 Sets. Z-scores denote the relative gene expression levels with red and violet representing high and low expression, respectively. (c) 2-way Venn diagram analysis of numbers of strain-biased adrenal DEGs identified from the comparisons of Set 1 (KK-M vs. C57-M: violet) and Set 3 (KK-F vs. C57-F: green), (FC≥± 1.4, FDR < 0.05). Green arrows represent up-regulated in KK-HlJ compared to C57Bl-6 J, and red arrows represent down-regulated. (d) 2-way Venn diagram analysis of numbers of sex-biased adrenal DEGs identified from the comparisons of Set 2 (KK-M vs. KK-F: yellow) and Set 4 (C57-M vs. C57-F: pink), (FC≥± 1.4, FDR < 0.05)