Fig. 5

GhARF2b affects fiber length and gene expression in RDL1::GhARF2b RNAi transgenic cotton. a Fiber phenotype of RDL1::GhARF2b (OE), RDL1::GhARF2b RNAi (ds) and wild-type (R15) cotton cultivated in farm on shanghai, bar = 10 mm. b Statistical analysis of RDL1::GhARF2b RNAi (ds) lines and wild-type (WT) mature fiber length. Error bar indicates standard deviation; *** denotes significant difference from wild type (Student’s t-test, P < 0.001, n = 30). c Expression of GhARF2b in 0 dpa ovules in the overexpression (OE), RNAi (ds) lines compared to wild-type (WT). d Expression of GhARF2b in 6-dpa fibers in the overexpression (OE), RNAi (ds) lines compared to wild-type (WT). e Expression of GhARF2b in 12-dpa fibers in the overexpression (OE) and the RNAi (ds) lines compared to the wild-type (WT). Error bar indicates stdev.s level of three qRT-PCR assays