Fig. 5

RT-qPCR validations of selected differentially expressed microRNAs in the livers of lifestyle modification mice models. The relative expression level of microRNAs in livers of CR (a), EX (b) and HF (c) were detected by PT-qPCR and normalized to U6, and the expression levels of AL mice were set at a relative expression of 1. RT-qPCR data were represented as mean ± SE and compared with microarray results. (*: p < 0.05, **: p < 0.01 and *** p < 0.001 vs AL. n = 5 in each group) AL: ad libitum, CR: caloric restriction, EX: exercise, HF: high-fat diet