Fig. 4

RNAseq comparison of OSN_eGFP+ to both MVC_eGFP and OSN_eGFP- cells. a Heatmap showing the top upregulated genes (excluding Olfrs) that are expressed in OSN_eGFP+ cells 4 fold higher than OSN_eGFP- AND MVC_eGFP cells. Additional criteria for inclusion was mean of expression > standard deviation of expression and mean of expression greater than 100. b Heatmap showing all Olfr genes differentially expressed between OSN_eGFP+ and OSN_eGFP- cells identified by DESeq2. MVC_eGFP cells did not express Olfrs. For both a and b, row and column order were determined automatically by the pHeatmap package in R. For each data point relative expression was calculated by subtracting the average row value from each individual value. c Volcano plot of all Olfactory receptors, demonstrating the small number of enriched olfactory receptors in the OSN_eGFP+ population. d Hierarchical clustering of transcripts for taste transduction and transcripts expressed in canonical and non-canonical OSNs identified by RNAseq as significantly different in expression between the cell groups. We compared expression of transcripts involved in taste transduction, canonical olfactory transduction, and non-canonical OSNs. The non-canonical OSNs considered here included guanilyl-cyclase D (GC-D) OSNs [37], Trpc2 OSNs [64], Cav2.1 OSNs [72], and OSNs expressing trace amine-associated receptors (Taars) [46]. Transcripts identified by DESeq2