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Table 4 Expression of mTOR pathway-related proteins in liver tissue from 4-d old Holstein calves (n = 6/group) born to cows randomly assigned to receive a basal control (CON) diet from − 28 ± 2 d to parturition [1.47 Mcal/kg dry matter (DM) and 15.3% crude protein (CP)] with no added Met or CON plus ethyl cellulose Met (MET, Mepron®, Evonik Nutrition & Care GmbH, Germany)

From: Unique adaptations in neonatal hepatic transcriptome, nutrient signaling, and one-carbon metabolism in response to feeding ethyl cellulose rumen-protected methionine during late-gestation in Holstein cows

Protein (log-transformed data) Maternal diet SEM p-value
CON MET
Total protein
 mTOR 0.93 0.90 0.08 0.85
 S6K1 2.55 2.38 0.10 0.43
 AKT 2.49 1.48 0.19 <.00
 4EBP1 2.39 2.42 0.17 0.93
 eEF2 1.91 1.69 0.09 0.26
 eIF2a 2.31 2.05 0.08 0.12
 RPS6 2.07 2.33 0.21 0.57
Phosphorylated protein
 p-mTOR 0.62 0.69 0.06 0.59
 p-S6K1 2.61 1.62 0.18 <.00
 p-AKT 2.46 2.24 0.11 0.36
 p-4EBP1 1.50 1.58 0.10 0.72
 p-eEF2 0.98 0.59 0.16 0.25
 p-eIF2a 2.41 2.08 0.15 0.29
 p-RPS6 1.78 1.17 0.21 0.15
Ratio
 p-mTOR/mTOR 0.74 0.85 0.12 0.69
 p-S6K1/S6K1 1.04 0.70 0.07 0.01
 p-AKT/AKT 1.01 1.52 0.09 <.00
 p-4EBP1/4EBP1 0.65 0.71 0.07 0.66
 p-eEF2/eEF2 0.51 0.36 0.08 0.35
 p-eIF2a/eIF2a 1.04 1.01 0.05 0.73
 p-RPS6/RPS6 0.90 0.61 0.13 0.28