Unique adaptations in neonatal hepatic transcriptome, nutrient signaling, and one-carbon metabolism in response to feeding ethyl cellulose rumen-protected methionine during late-gestation in Holstein cows

Table 7 Abundance of genes related to methionine metabolism, DNA methylation, glutathione metabolism, and cytidine 5′-diphosphocholine (CDP)–choline pathway in liver tissue from 4-d old Holstein calves (n = 6/group) born to cows randomly assigned to receive a basal control (CON) diet from − 28 ± 2 d to parturition [1.47 Mcal/kg dry matter (DM) and 15.3% crude protein (CP)] with no added Met or CON plus ethyl cellulose Met (MET, Mepron®, Evonik Nutrition & Care GmbH, Germany)

Gene (log₂ scale)	Maternal diet		SEM	p-value
Gene (log₂ scale)	CON	MET	SEM	p-value
*Methionine cycle*
MAT1A	0.01	0.03	0.01	0.09
BHMT	1.23	0.98	0.11	0.26
BHMT2	0.06	0.11	0.04	0.33
MTR	0.73	1.04	0.11	0.19
SAHH	0.04	0.10	0.01	0.53
PEMT	0.09	0.17	0.02	0.07
BADH	1.16	1.20	0.06	0.73
CHDH	1.57	1.50	0.15	0.76
SARDH	0.93	0.83	0.06	0.40
*DNA methylation*
DNMT1	0.95	0.94	0.03	0.93
DNMT3A	0.97	0.88	0.05	0.36
*Transsulfuration*
CBS	0.76	0.73	0.05	0.81
CDO1	0.91	0.74	0.05	0.08
CSAD	0.34	0.30	0.02	0.37
CTH	1.18	1.45	0.09	0.20
*Glutathione pathway*
GSS	1.02	0.97	0.03	0.48
GCLC	0.50	0.52	0.06	0.95
GSR	0.61	0.70	0.04	0.27
GPX1	0.86	1.02	0.06	0.13
*CDP–choline pathway*
CHKA	0.75	0.96	0.10	0.31
CHKB	0.76	0.76	0.03	0.94
PCYT1A	0.94	0.98	0.05	0.66
PCYT1B	0.82	0.50	0.08	0.04
CEPT1	0.94	0.96	0.05	0.92

ISSN: 1471-2164