Fig. 3

Inter-strain genomic variation in three average nucleotide identity (ANI)-defined species of Streptomyces (labeled as species 20, 29 and 38 in Fig. 2). a Core genome tree of each species. The number of core genes is 6591, 5125 and 5979 for species 20, 29 and 38, respectively. Scale bars represent the number of nucleotide substitutions per site. b Strain distribution of genes in the pangenome of each species. c Strain distribution of biosynthetic gene clusters (BGCs) in each species. Hybrid BGCs were classified according to their BGC components. Acronyms: AT – acyltransferase; CDPS – tRNA-dependent cyclodipeptide synthases; hgIE-KS – heterocyst glycolipid synthase-like ketide synthase; NRPS – non-ribosomal peptide synthetase; PKS – polyketide synthase. Comparison of the d total number of genes, e accessory genes and f singleton genes in the pan-genome of each species. * denotes a p-value < 0.05. For visual clarity, comparisons that were not statistically significant are not shown. Significance measured using Mann-Whitney U pairwise test. Box plots depict the minimum, first quartile, median, third quartile, and maximum values