Fig. 4

Conjoint analysis m6A-seq and RNA-seq data. a Negative correlation between overall m6A methylation and mRNA expression level (r = −0.3651; y = −0.30x + 0.27). b Distribution of genes with a significant change in both m6A methylation level and gene expression between E30 and E20 (DMGs). Hyper-up, Hyper-down represent genes with increased m6A methylation level and increased gene expression, genes with increased m6A methylation level and decreased gene expression, respectively. Hypo-up, Hypo-down represent genes with decreased m6A methylation level and increased gene expression, genes with decreased m6A methylation level and decreased gene expression, respectively. c Expression levels of genes carrying different number of m6A peaks in E21. d Expression levels of genes carrying different number of m6A peaks in E30. e The m6A modification sites and gene expression in all m6A peaks, E21-unique peaks and E30-unique peaks. f Heat map of 26 muscle related genes represented in Table 1