Fig. 5

Comparison of regular RNA-seq and Cappable-seq in global transcriptional analyses. a Correlation between fold-changes of RNA levels and TSS strength under hypoxia (H) compared with normoxia (N) from regular RNA-seq and Cappable-seq data. The graph containing 2387 pTSSs expressed under both normoxia and hypoxia and the expression of downstream genes of these pTSSs were detected by regular RNA-seq. The red and blue dots indicate 393 upregulated and 175 downregulated genes that probably subjected to posttranscriptional regulation. The fad5A gene was chosen for verification using qRT-PCR. b and c Transcriptional (b) and translational (c) fusions of fadA5 to egfp. The primers for qRT-PCR were designed according to the egfp sequence. Relative transcriptional levels under normoxic conditions were normalized to 1. Data are shown as the mean ± SEM values from biological triplicates performed in duplicate. *, p < 0.05; n.s., not significant