Fig. 4

Comparison of the relative contents of chlorophyll precursors. Three individuals were measured for each chlorophyll precursor. Error bars indicate means ± SD based on three independent experiments. Significant differences were determined using the Student’s t test in WY16–13 compared with W16–13 (^* p < 0.05, ^** p < 0.01). Glu, Glutamate; ALA, 5-aminolevulinic acid; PBG, porphobilinogen; Urogen III, uroporphyrinogen III; Coprogen III, coproporphyrinogen III; Proto IX, protoporphyrin IX; Mg-Proto IX, Mg-protoporphyrin IX; Pchlide, protochlorophyllide