Fig. 5

Aging-related cellular phenotypes at various organs. a–d Representative morphologies of abnormal mitochondria and vacuoles containing remnants in aged flight muscle. The yellow triangles indicate normal mitochondria, whereas the red triangles indicate abnormal mitochondria or vacuoles. Bar length = 1 μm. e Percentages of abnormal mitochondria and vacuoles containing remnants shown in (a–d) at 14, 21, and 28 D. The bar graphs with quantifications belong to the abnormity on the same line. The values are expressed as mean ± S.E.M. Significant differences are denoted by different letters (one-way ANOVA, P < 0.05). f Morphological characteristics of fat body cells at 14, 21, and 28 D. Blue, Hoechest 33,342 (cell nuclei); green, phalloidin (F-actin). Bar length = 50 μm. (G,H) Apoptotic cell detection in fat body (g) and brain (h) at 14, 21, and 28 D. Blue, Hoechest 33,342 (cell nuclei); green, TdT-mediated dUTP nick end labeling (TUNEL) (apoptotic cells). Bar length = 200 μm. The statistical analyses of abnormal nuclei shown in (f, g, h) are in Fig. S5