Fig. 3

Normalization and exploration of RNA-seq dataset with DIANE. a PCA analysis for the normalized expression table. The experimental conditions have for coordinates their contributions (correlations) to the first four principal components. The scree-plot shows, for each principal component, the part of global variability explained. b Example of normalized gene expression levels across all seven perturbations and control. c MA-plot for the DEG in response to a single heat stress. The x-axis is the average expression, and the y-axis is the LFC in expression between heat stress and control. DEG with FDR <0.05 and an absolute LFC >2 appear in green. d Log normalized expression heatmap for the DEG under heat across all perturbations and control