Table 1 Raw read data summary for unfiltered, Albacore “passed” and filtered Oxford Nanopore long-read sequencing datasets for Thalassiosira pseudonana and Phaeodactylum tricornutum. The unfiltered data include all sequence reads, including passed (q-score>7) and failed (q-score<7) reads as determined by Albacore. The Albacore “pass” data include all reads with a quality-score >7. The filtered datasets for T. pseudonana and P. tricornutum included reads with read length ≥30 kb and ≥20 kb, respectively
From: Re-examination of two diatom reference genomes using long-read sequencing
Unfiltered Data | Albacore “pass” Data | Filtered Data | ||
|---|---|---|---|---|
Phaeodactylum tricornutum | Total bases (Gbp) | 8.2 | 7.5 | 2.7 |
No. of reads | 986,604 | 820,187 | 84,445 | |
Mean read length (bp) | 8,311.1 | 9,144.7 | 31,973.9 | |
Mean read quality | 8.5 | 9.2 | 9.6 | |
Read length N50 (bp) | 18,756 | 19,261 | 32,648 | |
Estimated genome coverage | ~300x | ~273x | ~100x | |
Percentage of reads mapped to JGI reference | 76.8% | 87.8% | 92.6% | |
Average percent identity of reads to JGI reference | 73.7% | |||
Thalassiosira pseudonana | Total bases (Gbp) | 7.5 | 7.0 | 1.8 |
No. of reads | 701,596 | 580,845 | 46,708 | |
Mean read length (bp) | 10,611.8 | 12,029.7 | 37,942.3 | |
Mean read quality | 9.9 | 10.8 | 10.9 | |
Read length N50 (bp) | 20,088 | 20,514 | 37,303 | |
Estimated genome coverage | ~230x | ~215x | ~50x | |
Percentage of reads mapped to JGI reference | 76.6% | 89.1% | 93.5% | |
Average percent identity of reads to JGI reference | 71.5% |