Fig. 5

Temporal and spatial expression dynamics of SsR2R3-MYB genes. a A heatmap showed the expression profile of SsR2R3-MYB genes. Columns showed 202 SsR2R3-MYB genes, and rows showed developmental stages and tissues, leaf developmental gradient, and circadian rhythm. Abbreviations: s, seeding stage; pm, pre-mature stage; m, mature stage; r leaf, roll leaf; m leaf, mature leaf. Leaf developmental gradient showed 1–15 segment in one leaf blade from base to tip. Circadian rhythm showed 19-time point including first day 2 hours period and second day 4 hours period. Asterisk was used to distinguish the same time point on different days. b K-means clustering showing the expression profile of the developmental stages and tissue transcriptome. Five clusters were identified as C1-C5, error bars showing standard deviation. c Heatmap of gene-expression levels for co-expression modules along leaf developmental. Each gene (row) in the two co-expression modules (I, II) was sorted according to the point of leaf segment point (column) at which peak expression occurred. d The expression of nine MYB genes showed a circadian cycle, and the x-axis indicates different time points on the second day