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Table 15 The Knockdown Percentage (KD%) calculation for the RNA-Seq validation method

From: Functional prediction of de novo uni-genes from chicken transcriptomic data following infectious bursal disease virus at 3-days post-infection

Step 1

Normalize to (REF): ∆Cq = Cq (TAR) – Cq (REF)

Step 2

Exponential expression transform: ∆Cq Expression = 2–∆Cq

Step 3

Averages replicates and calculate standard deviation

Step 4

Normalize to treatment control

Step 5. % KD = (1 – ∆∆Cq) × 100

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BMC Genomics

ISSN: 1471-2164

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