Fig. 3

Vole brain gene expression varies by species. a Volcano plot showing significance (−log₁₀(p-value)) and magnitude of difference (log₂ fold change, LFC) in expression for each gene in contrast between virgin prairie and meadow voles. Each point represents a single gene. Orange colored points pass significance threshold of FDR < 0.1. Darker shaded points pass FDR cutoff and have LFC > 2. Positive LFC values represent genes more highly expressed in prairie voles, negative LFC values are genes more highly expressed in meadow voles. b Enriched GO terms for contrasts between prairie and meadow voles. Hierarchical clustering tree shows relationship between GO categories based on shared genes. Branches with length of zero are subsets of one another. Fractions preceding GO terms indicate proportion of “good” genes that have raw p-value< 0.05 compared to total number of genes in the category. Bold text indicates adjusted p < 0.01, plain text indicates adjusted p < 0.05, and italicized text indicates adjusted p < 0.1 for term. P-values are corrected using Benjamini-Hochberg false discovery rate procedure [35]. Red terms are enriched in prairie voles and blue terms are enriched in meadow voles