Fig. 2From: Chromatin accessibility profiling in Neurospora crassa reveals molecular features associated with accessible and inaccessible chromatinChromatin accessibility is associated with nucleosome turnover and transcription factor binding. (A) The Western blot shows induction of an ectopic H3-3xFLAG construct under the control of a copper-regulated promoter to measure integration of new H3 (i.e. hH3 turnover). Protein levels are shown at 0, 1, 2, 3, 4, and 8 h after induction using the copper chelator BCS. (B) The genome browser image for the left arm of Linkage Group 1 (LGI-Left Arm) illustrates the overlapping patterns of accessible chromatin (blue) and newly incorporated H3-3xFLAG (green). (C) The heatmap shows global overlap of chromatin accessibility and histone turnover measured by H3-3xflag enrichment following induction. The heatmaps are centered on ACRs and ordered by peak size. (D) Representative genome browser images of the frq locus and vvd locus illustrate ATAC-seq enrichment patterns in WT and the Δwc-2 mutant. (E) The heatmap shows enrichment data across all WC2-binding sites for the indicated experiments. Previously published WC-2 ChIP-seq enrichment is shown for the wild-type strain. ATAC-seq enrichment is shown for wild type and the Δwc-2 mutantBack to article page