Fig. 1

Relationship between the parental cell line BEF2, edited cell clone CC14 and edited and control calves. Shown is an experimental flow diagram from the parental cell line BEF2 to the two coat colour-diluted Holstein-Friesian calves homozygous for PMEL p.Leu18del and three wild-type control calves. A subset of the male primary bovine fetal fibroblasts (BEF2) were transfected with a plasmid-encoded, PMEL-specific editor and a single stranded homology directed repair (HDR) template. Post transfection, a single mitotic doublet was used for the clonal isolation of CC14 with a homozygous PMEL p.Leu18del mutation. Two edited cloned calves (1805 and B071) and three non-edited control calves (1802, 1803 and 1804) were generated via somatic cell nuclear transfer using CC14 and BEF2 as donor cells, respectively. The ‘named’ samples are those that were sequenced in this study (i.e., BEF2, CC14, 1802, 1803, 1804, 1805, and B071)