Fig. 5
From: Stage-specific transcriptomic changes in pancreatic α-cells after massive β-cell loss
Ifit3 expression is strongly induced in α-cells after acute β-cell loss. A Representative immunofluorescence staining of pancreas sections obtained from Ctrl, 5dpDT and 15dpDT treated mice. B Percentage of cells co-expressing Ifit3 and YFP, expressed relative to the total number of total YFP+ cells in the islets. * P < 0.05. Total number of YFP+ cells (expressed as mean ± SEM) counted per replicate: Control (615 ± 268), 5dpDT (341 ± 184), 15dpDT (206 ± 48) from 3 biological replicates. C Percentage of cells expressing YFP, Ifit3, and co-expressing both markers, expressed relative to the total number of islet cells. Note that YFP+ cells represent the main cell type in 15dpDT islets given that by this timepoint β-cell ablation is almost complete. ** P < 0.01. Total number of islet cells (expressed as mean ± SEM) counted per replicate: Control (2291 ± 613), 5dpDT (1110 ± 455), 15dpDT (404 ± 96) from 3 biological replicates