Fig. 2

Characterization of the venom proteins of H. curtus from South China Sea. Three milligrams of total venom were applied to a C18 column, and separated as described on Materials and methods. Fractions were collected manually and submitted to molecular determination by SDS-PAGE under reduced conditions (original images were listed in Figure S1). Protein bands were excised, tryptic digested and analyzed by MALDI-TOF/TOF or nESI-MS/MS for their assignment to known protein families. The results are shown in Table 1