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Table 1 Assignment of the chromatographic fractions and electrophoretic bands from H. curtus venom to protein families

From: Venom-gland transcriptomic, venomic, and antivenomic profiles of the spine-bellied sea snake (Hydrophis curtus) from the South China Sea

Peak

%

MW

(kDa)

Peptide Ion

MS/MS-derived sequence

Protein family/species/accession/transcript ID

m/z

z

1

33.13

12.9

401.2

2

TWSDHR

3-FTx (SNX); Hydrophis schistosus; P68415; Hcu|50480

650.8

2

GCGCPBVBPGXB

669.3

2

TCCNBBSSBPB

711.3

2

TTTNCAESSCYB

734.8

2

MTCCNBBSSBPB

742.8

2

MTCCNBBSSBPB

517.2

3

TTTNCAESSCYBB

775.3

2

TTTNCAESSCYBB

2

3.05

13.3

1275.5

1

SWCDAFCSSR

3-FTx (LNX); Hydrophis hardwickii; A3FM53; Hcu|53057

2190.0

1

THPYBPETCPPGBNXCYB

 

1.56

11.5

1275.5

1

SWCDAFCSSR

3-FTx (LNX); H. hardwickii; A3FM53; Hcu|54185

1403.6

1

BSWCDAFCSSR

2055.9

1

DXNCCATDNCNTVANWB

2190.0

1

THPYBPETCPPGBNXCYB

3

1.39

13.8

1245.5

1

SWCDAFCGSR

3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057

 

0.90

12.0

1245.5

1

SWCDAFCGSR

3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057

4

0.14

16.8

1245.5

1

SWCDAFCGSR

3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057

 

0.03

14.7

1245.5

1

SWCDAFCGSR

3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057

 

0.04

13.8

1275.5

1

SWCDAFCSSR

3-FTx (LNX); H. hardwickii; Q8UW29; Hcu|51842

1403.6

1

BSWCDAFCSSR

2053.0

1

TPYBPETCPPGBNXCYB

 

0.13

12.3

1275.5

1

SWCDAFCSSR

3-FTx (LNX); H. hardwickii; A3FM53; Hcu|54185

1403.6

1

BSWCDAFCSSR

2055.9

1

DXNCCATDNCNTVANWB

2190.0

1

THPYBPETCPPGBNXCYB

5

1.05

25.3

1336.6

1

XHDDCYGEAEB

PLA2; H. schistosus; P00610; Hcu|52733

1514.7

1

NAYNNANYNXDTB

1878.9

1

NXVBFSYVXTCANHNR

1936.0

1

NXVBFSYVXTCANHNR

2785.2

1

SSXDYADYGCYCGAGGSGTPVDEXDR

 

0.33

21.1

1336.6

1

XHDDCYGEAEB

PLA2; H. schistosus; P00610; Hcu|52733

1514.7

1

NAYNNANYNXDTB

1878.9

1

NXVBFSYVXTCANHNR

1936.0

1

NXVBFSYVXTCANHNR

2069.9

1

XHDDCYGEAEBBGCYPB

2785.2

1

SSXDYADYGCYCGAGGSGTPVDEXDR

 

1.14

16.9

1336.6

1

XHDDCYGEAEB

PLA2; H. schistosus; P00610; Hcu|52733

1514.7

1

NAYNNANYNXDTB

1878.9

1

NXVBFSYVXTCANHNR

2785.1

1

SSXDYADYGCYCGAGGSGTPVDEXDR

 

0.15

14.8

1336.6

1

XHDDCYGEAEB

PLA2; H. hardwickii; Q8UW30; Hcu|52733

1514.7

1

NAYNNANYNXDTB

1955.8

1

MXXYDYDCGSNGPYCB

2785.2

1

SSXDYADYGCYCGAGGSGTPVDEXDR

 

0.27

13.9

1275.5

1

SWCDAFCSSR

3-FTx (LNX); H. hardwickii; Q8UW29; Hcu|51842

1403.6

1

BSWCDAFCSSR

2053.0

1

TPYBPETCPPGBNXCYB

 

1.16

12.6

1275.5

1

SWCDAFCSSR

3-FTx (LNX); H. hardwickii; Q8UW29; Hcu|51395

6

1.56

19.1

1514.7

1

NAYNNANYNXDTB

PLA2; H. schistosus; P00610; Hcu|52733

1879.0

1

NXVBFSYVXTCANHNR

1936.0

1

NXVBFSYVXTCANHNR

 

8.34

16.9

1336.6

1

XHDDCYGEAEB

PLA2; H. schistosus; P00610; Hcu|52499

1514.7

1

NAYNNANYNXDTB

1878.9

1

NXVBFSYVXTCANHNR

1936.0

1

NXVBFSYVXTCANHNR

2785.1

1

SSXDYADYGCYCGAGGSGTPVDEXDR

7

0.33

18.9

1336.6

1

XHDDCYGEAEB

PLA2; H. schistosus; P00610; Hcu|52733

1514.7

1

NAYNNANYNXDTB

1936.0

1

NXVBFSYVXTCANHNR

 

0.25

16.4

1336.6

1

XHDDCYGEAEB

PLA2; H. schistosus; P00610; Hcu|52733

1514.7

1

NAYNNANYNXDTB

1879.0

1

NXVBFSYVXTCANHNR

1936.0

1

NXVBFSYVXTCANHNR

2785.2

1

SSXDYADYGCYCGAGGSGTPVDEXDR

 

0.12

14.5

1936.0

1

NXVBFSYVXTCANHNR

PLA2; H. schistosus; P00610; Hcu|52837

 

0.15

12.0

1245.5

1

SWCDAFCGSR

3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057

8

19.26

16.1

528.2

2

AFXCNCDR

PLA2; H. hardwickii; Q8UW31; Hcu|52837

429.9

3

NMXBCANHGSR

644.3

2

NMXBCANHGSR

435.2

3

NMXBCANHGSR

652.3

2

NMXBCANHGSR

668.8

2

XHDDCYGEAEB

692.3

2

TAAXCFAGAPYNB

644.3

3

DNNDECBAFXCNCDR

965.9

2

DNNDECBAFXCNCDR

829.7

3

TAAXCFAGAPYNBENYNXDXNB

986.4

3

MTXDYMDYGCYCGTGGSGTPVDEXDR

 

5.03

15.4

1055.5

1

AFXCNCDR

PLA2; H. hardwickii; Q8UW31; Hcu|52837

1383.7

1

TAAXCFAGAPYNB

 

10.67

14.4

1383.7

1

TAAXCFAGAPYNB

PLA2; H. hardwickii; Q8UW31; Hcu|52515

9

0.31

73.0

561.8

2

ENYNXDXNB

PLA2; H. hardwickii; Q8UW31; Hcu|52733

587.3

2

MXBCANHGSR

516.6

3

ENYNXDXNBHCB

429.9

3

NMXBCANHGSR

652.3

2

NMXBCANHGSR

692.3

2

TAAXCFAGAPYNB

809.4

2

XHDDCYGEAEBXPA

680.8

2

GTGGSGTPVDEXDR

760.8

2

CGTGGSGTPVDEXDR

694.3

3

NXYBFBNMXBCANHGSR

699.7

3

NXYBFBNMXBCANHGSR

829.7

3

TAAXCFAGAPYNBENYNXDXNB

978.4

3

MTXDYMDYGCYCGTGGSGTPVDEXDR

983.7

3

MTXDYMDYGCYCGTGGSGTPVDEXDR

983.7

3

MTXDYMDYGCYCGTGGSGTPVDEXDR

1483.1

2

MTXDYMDYGCYCGTGGSGTPVDEXDR

1092.0

4

XPACNYMXSGPYYNXYTYDCVEHBXTCBDNNDECB

962.2

5

XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB

 

0.29

15.9

1055.5

1

AFXCNCDR

PLA2; H. hardwickii; Q8UW31; Hcu|52733

1383.7

1

TAAXCFAGAPYNB

 

0.19

14.6

528.2

2

AFXCNCDR

PLA2; H. hardwickii; Q8UW31; Hcu|52837

561.8

2

ENYNXDXNB

644.3

2

NMXBCANHGSR

429.9

3

NMXBCANHGSR

652.3

2

NMXBCANHGSR

435.2

3

NMXBCANHGSR

668.8

2

XHDDCYGEAEB

692.3

2

TAAXCFAGAPYNB

774.3

2

ENYNXDXNBHCB

595.6

3

CCBXHDDCYGEAEB

965.9

2

DNNDECBAFXCNCDR

829.7

3

TAAXCFAGAPYNBENYNXDXNB

978.4

3

MTXDYMDYGCYCGTGGSGTPVDEXDR

1467.1

2

MTXDYMDYGCYCGTGGSGTPVDEXDR

989.1

3

MTXDYMDYGCYCGTGGSGTPVDEXDR

1475.1

2

MTXDYMDYGCYCGTGGSGTPVDEXDR

1158.5

3

XPACNYMXSGPYYNXYTYDCVEHBXTCB

1163.8

3

XPACNYMXSGPYYNXYTYDCVEHBXTCB

1092.0

4

XPACNYMXSGPYYNXYTYDCVEHBXTCBDNNDECB

959.0

5

XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB

1198.5

4

XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB

10

0.33

16.0

561.8

2

ENYNXDXNB

PLA2; H. hardwickii; Q8UW31; Hcu|52837

644.3

2

NMXBCANHGSR

435.2

3

NMXBCANHGSR

652.3

2

NMXBCANHGSR

668.8

2

XHDDCYGEAEB

692.3

2

TAAXCFAGAPYNB

516.6

3

ENYNXDXNBHCB

774.3

2

ENYNXDXNBHCB

644.3

3

DNNDECBAFXCNCDR

965.9

2

DNNDECBAFXCNCDR

829.7

3

TAAXCFAGAPYNBENYNXDXNB

728.8

4

TAAXCFAGAPYNBENYNXDXNBHCB

971.4

3

TAAXCFAGAPYNBENYNXDXNBHCB

983.7

3

MTXDYMDYGCYCGTGGSGTPVDEXDR

989.0

3

MTXDYMDYGCYCGTGGSGTPVDEXDR

1475.0

2

MTXDYMDYGCYCGTGGSGTPVDEXDR

1092.0

4

XPACNYMXSGPYYNXYTYDCVEHBXTCBDNNDECB

962.2

5

XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB

1202.5

4

XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB

11

4.21

27.1

964.5

1

CBTEWXB

CRISP; H. hardwickii; Q8UW11; Hcu|52188

1092.6

1

BCBTEWXB

1126.6

1

WNSHAABNAB

1194.7

1

EXVDBHNAXR

1342.6

1

CTFAHSPEHTR

1509.6

1

SBCPATCFCHNB

1776.8

1

YXYVCBYCPAGNXR

 

0.55

18.5

1342.6

1

CTFAHSPEHTR

CRISP; H. hardwickii; Q8UW11; Hcu|52188

1776.8

1

YXYVCBYCPAGNXR

 

0.74

17.2

1342.6

1

CTFAHSPEHTR

CRISP; H. hardwickii; Q8UW11; Hcu|52570

 

3.20

16.1

1776.8

1

YXYVCBYCPAGNXR

CRISP; H. hardwickii; Q8UW11; Hcu|52188

  1. X: Leu/Ile; B: Lys/Gln. Methionine oxidation is underlined
  2. Transcripts are listed in the Additional Table S2
  3. 3-FTx three finger toxin, PLA2 phospholipase A2, CRISP cysteine-rich secretory protein, LNX long chain α-neurotoxin; SNX, short chain α-neurotoxin
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