Skip to main content

Table 1 Assignment of the chromatographic fractions and electrophoretic bands from H. curtus venom to protein families

From: Venom-gland transcriptomic, venomic, and antivenomic profiles of the spine-bellied sea snake (Hydrophis curtus) from the South China Sea

Peak % MW
(kDa)
Peptide Ion MS/MS-derived sequence Protein family/species/accession/transcript ID
m/z z
1 33.13 12.9 401.2 2 TWSDHR 3-FTx (SNX); Hydrophis schistosus; P68415; Hcu|50480
650.8 2 GCGCPBVBPGXB
669.3 2 TCCNBBSSBPB
711.3 2 TTTNCAESSCYB
734.8 2 MTCCNBBSSBPB
742.8 2 MTCCNBBSSBPB
517.2 3 TTTNCAESSCYBB
775.3 2 TTTNCAESSCYBB
2 3.05 13.3 1275.5 1 SWCDAFCSSR 3-FTx (LNX); Hydrophis hardwickii; A3FM53; Hcu|53057
2190.0 1 THPYBPETCPPGBNXCYB
  1.56 11.5 1275.5 1 SWCDAFCSSR 3-FTx (LNX); H. hardwickii; A3FM53; Hcu|54185
1403.6 1 BSWCDAFCSSR
2055.9 1 DXNCCATDNCNTVANWB
2190.0 1 THPYBPETCPPGBNXCYB
3 1.39 13.8 1245.5 1 SWCDAFCGSR 3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057
  0.90 12.0 1245.5 1 SWCDAFCGSR 3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057
4 0.14 16.8 1245.5 1 SWCDAFCGSR 3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057
  0.03 14.7 1245.5 1 SWCDAFCGSR 3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057
  0.04 13.8 1275.5 1 SWCDAFCSSR 3-FTx (LNX); H. hardwickii; Q8UW29; Hcu|51842
1403.6 1 BSWCDAFCSSR
2053.0 1 TPYBPETCPPGBNXCYB
  0.13 12.3 1275.5 1 SWCDAFCSSR 3-FTx (LNX); H. hardwickii; A3FM53; Hcu|54185
1403.6 1 BSWCDAFCSSR
2055.9 1 DXNCCATDNCNTVANWB
2190.0 1 THPYBPETCPPGBNXCYB
5 1.05 25.3 1336.6 1 XHDDCYGEAEB PLA2; H. schistosus; P00610; Hcu|52733
1514.7 1 NAYNNANYNXDTB
1878.9 1 NXVBFSYVXTCANHNR
1936.0 1 NXVBFSYVXTCANHNR
2785.2 1 SSXDYADYGCYCGAGGSGTPVDEXDR
  0.33 21.1 1336.6 1 XHDDCYGEAEB PLA2; H. schistosus; P00610; Hcu|52733
1514.7 1 NAYNNANYNXDTB
1878.9 1 NXVBFSYVXTCANHNR
1936.0 1 NXVBFSYVXTCANHNR
2069.9 1 XHDDCYGEAEBBGCYPB
2785.2 1 SSXDYADYGCYCGAGGSGTPVDEXDR
  1.14 16.9 1336.6 1 XHDDCYGEAEB PLA2; H. schistosus; P00610; Hcu|52733
1514.7 1 NAYNNANYNXDTB
1878.9 1 NXVBFSYVXTCANHNR
2785.1 1 SSXDYADYGCYCGAGGSGTPVDEXDR
  0.15 14.8 1336.6 1 XHDDCYGEAEB PLA2; H. hardwickii; Q8UW30; Hcu|52733
1514.7 1 NAYNNANYNXDTB
1955.8 1 MXXYDYDCGSNGPYCB
2785.2 1 SSXDYADYGCYCGAGGSGTPVDEXDR
  0.27 13.9 1275.5 1 SWCDAFCSSR 3-FTx (LNX); H. hardwickii; Q8UW29; Hcu|51842
1403.6 1 BSWCDAFCSSR
2053.0 1 TPYBPETCPPGBNXCYB
  1.16 12.6 1275.5 1 SWCDAFCSSR 3-FTx (LNX); H. hardwickii; Q8UW29; Hcu|51395
6 1.56 19.1 1514.7 1 NAYNNANYNXDTB PLA2; H. schistosus; P00610; Hcu|52733
1879.0 1 NXVBFSYVXTCANHNR
1936.0 1 NXVBFSYVXTCANHNR
  8.34 16.9 1336.6 1 XHDDCYGEAEB PLA2; H. schistosus; P00610; Hcu|52499
1514.7 1 NAYNNANYNXDTB
1878.9 1 NXVBFSYVXTCANHNR
1936.0 1 NXVBFSYVXTCANHNR
2785.1 1 SSXDYADYGCYCGAGGSGTPVDEXDR
7 0.33 18.9 1336.6 1 XHDDCYGEAEB PLA2; H. schistosus; P00610; Hcu|52733
1514.7 1 NAYNNANYNXDTB
1936.0 1 NXVBFSYVXTCANHNR
  0.25 16.4 1336.6 1 XHDDCYGEAEB PLA2; H. schistosus; P00610; Hcu|52733
1514.7 1 NAYNNANYNXDTB
1879.0 1 NXVBFSYVXTCANHNR
1936.0 1 NXVBFSYVXTCANHNR
2785.2 1 SSXDYADYGCYCGAGGSGTPVDEXDR
  0.12 14.5 1936.0 1 NXVBFSYVXTCANHNR PLA2; H. schistosus; P00610; Hcu|52837
  0.15 12.0 1245.5 1 SWCDAFCGSR 3-FTx (LNX); H. hardwickii; Q8UW28; Hcu|53057
8 19.26 16.1 528.2 2 AFXCNCDR PLA2; H. hardwickii; Q8UW31; Hcu|52837
429.9 3 NMXBCANHGSR
644.3 2 NMXBCANHGSR
435.2 3 NMXBCANHGSR
652.3 2 NMXBCANHGSR
668.8 2 XHDDCYGEAEB
692.3 2 TAAXCFAGAPYNB
644.3 3 DNNDECBAFXCNCDR
965.9 2 DNNDECBAFXCNCDR
829.7 3 TAAXCFAGAPYNBENYNXDXNB
986.4 3 MTXDYMDYGCYCGTGGSGTPVDEXDR
  5.03 15.4 1055.5 1 AFXCNCDR PLA2; H. hardwickii; Q8UW31; Hcu|52837
1383.7 1 TAAXCFAGAPYNB
  10.67 14.4 1383.7 1 TAAXCFAGAPYNB PLA2; H. hardwickii; Q8UW31; Hcu|52515
9 0.31 73.0 561.8 2 ENYNXDXNB PLA2; H. hardwickii; Q8UW31; Hcu|52733
587.3 2 MXBCANHGSR
516.6 3 ENYNXDXNBHCB
429.9 3 NMXBCANHGSR
652.3 2 NMXBCANHGSR
692.3 2 TAAXCFAGAPYNB
809.4 2 XHDDCYGEAEBXPA
680.8 2 GTGGSGTPVDEXDR
760.8 2 CGTGGSGTPVDEXDR
694.3 3 NXYBFBNMXBCANHGSR
699.7 3 NXYBFBNMXBCANHGSR
829.7 3 TAAXCFAGAPYNBENYNXDXNB
978.4 3 MTXDYMDYGCYCGTGGSGTPVDEXDR
983.7 3 MTXDYMDYGCYCGTGGSGTPVDEXDR
983.7 3 MTXDYMDYGCYCGTGGSGTPVDEXDR
1483.1 2 MTXDYMDYGCYCGTGGSGTPVDEXDR
1092.0 4 XPACNYMXSGPYYNXYTYDCVEHBXTCBDNNDECB
962.2 5 XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB
  0.29 15.9 1055.5 1 AFXCNCDR PLA2; H. hardwickii; Q8UW31; Hcu|52733
1383.7 1 TAAXCFAGAPYNB
  0.19 14.6 528.2 2 AFXCNCDR PLA2; H. hardwickii; Q8UW31; Hcu|52837
561.8 2 ENYNXDXNB
644.3 2 NMXBCANHGSR
429.9 3 NMXBCANHGSR
652.3 2 NMXBCANHGSR
435.2 3 NMXBCANHGSR
668.8 2 XHDDCYGEAEB
692.3 2 TAAXCFAGAPYNB
774.3 2 ENYNXDXNBHCB
595.6 3 CCBXHDDCYGEAEB
965.9 2 DNNDECBAFXCNCDR
829.7 3 TAAXCFAGAPYNBENYNXDXNB
978.4 3 MTXDYMDYGCYCGTGGSGTPVDEXDR
1467.1 2 MTXDYMDYGCYCGTGGSGTPVDEXDR
989.1 3 MTXDYMDYGCYCGTGGSGTPVDEXDR
1475.1 2 MTXDYMDYGCYCGTGGSGTPVDEXDR
1158.5 3 XPACNYMXSGPYYNXYTYDCVEHBXTCB
1163.8 3 XPACNYMXSGPYYNXYTYDCVEHBXTCB
1092.0 4 XPACNYMXSGPYYNXYTYDCVEHBXTCBDNNDECB
959.0 5 XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB
1198.5 4 XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB
10 0.33 16.0 561.8 2 ENYNXDXNB PLA2; H. hardwickii; Q8UW31; Hcu|52837
644.3 2 NMXBCANHGSR
435.2 3 NMXBCANHGSR
652.3 2 NMXBCANHGSR
668.8 2 XHDDCYGEAEB
692.3 2 TAAXCFAGAPYNB
516.6 3 ENYNXDXNBHCB
774.3 2 ENYNXDXNBHCB
644.3 3 DNNDECBAFXCNCDR
965.9 2 DNNDECBAFXCNCDR
829.7 3 TAAXCFAGAPYNBENYNXDXNB
728.8 4 TAAXCFAGAPYNBENYNXDXNBHCB
971.4 3 TAAXCFAGAPYNBENYNXDXNBHCB
983.7 3 MTXDYMDYGCYCGTGGSGTPVDEXDR
989.0 3 MTXDYMDYGCYCGTGGSGTPVDEXDR
1475.0 2 MTXDYMDYGCYCGTGGSGTPVDEXDR
1092.0 4 XPACNYMXSGPYYNXYTYDCVEHBXTCBDNNDECB
962.2 5 XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB
1202.5 4 XHDDCYGEAEBXPACNYMXSGPYYNXYTYDCVEHBXTCB
11 4.21 27.1 964.5 1 CBTEWXB CRISP; H. hardwickii; Q8UW11; Hcu|52188
1092.6 1 BCBTEWXB
1126.6 1 WNSHAABNAB
1194.7 1 EXVDBHNAXR
1342.6 1 CTFAHSPEHTR
1509.6 1 SBCPATCFCHNB
1776.8 1 YXYVCBYCPAGNXR
  0.55 18.5 1342.6 1 CTFAHSPEHTR CRISP; H. hardwickii; Q8UW11; Hcu|52188
1776.8 1 YXYVCBYCPAGNXR
  0.74 17.2 1342.6 1 CTFAHSPEHTR CRISP; H. hardwickii; Q8UW11; Hcu|52570
  3.20 16.1 1776.8 1 YXYVCBYCPAGNXR CRISP; H. hardwickii; Q8UW11; Hcu|52188
  1. X: Leu/Ile; B: Lys/Gln. Methionine oxidation is underlined
  2. Transcripts are listed in the Additional Table S2
  3. 3-FTx three finger toxin, PLA2 phospholipase A2, CRISP cysteine-rich secretory protein, LNX long chain α-neurotoxin; SNX, short chain α-neurotoxin