Fig. 2
Global gene expression profiles and differentially expressed genes (DEGs) during the formation of pellicle cysts. (a) Hierarchical cluster analysis (HCA) of DEGs in the CK, D2, D5 and PC (3 replicates for each sample); (b) Volcano plot of gene expression levels comparing CK to D5 showing the number of DEGs in red (up-regulated) and green (down-regulated), and those that are unchanged are in black; (c) Volcano plot of gene expression levels comparing CK to PC. The color scale represents the log2-transformed FPKM values. CK represents the control group of vegetative cells in the exponential phase; D2 and D5 represent the groups treated with cold and darkness for two hours and five hours, respectively; PC represents the group of pellicle cysts