Fig. 4

Western blot analysis of the effect of PND on EgMKK1 and EgMKK2. Protein expression levels of EgMKK1 and EgMKK2 in PSCs without (control, lane 1) or with the treatment of PND at the concentrations of LC₁₀ (9.9 µM, lane 2), LC₃₀ (30.6 µM, lane 3) and LC₅₀ (49.0 µM, lane 4, identical treatment to that of RNA-seq samples) for 24 h. *p < 0.05, **p < 0.01, compared to control