Table 2 The primers used in this study

I1–2

5,107,359

CCCTTGCACACTTATTTGACTAGT

CCATTGCTGTTTATATTCGGGTGG

232

0.5739

4.0000

0.5462

0.4635

I1–4

35,070,118

CGATACAATCTAAGGGGAGTAGGC

CTGAGAGTTAGCACCACCATTTTG

225

0.6261

4.0000

0.5072

0.4277

I1–6

55,100,664

TCAGTTAATAATCGCACGCACATC

GATCCTGGTTCGTGAAATTGATGG

215

0.4522

4.0000

0.6865

0.6351

I2–10

45,232,264

CTAACTAACCATCTACTGCGACCA

CTCTGGATCCATTTTCGTTTGAGG

217

0.4913

4.0000

0.6149

0.5407

I4–10

45,033,231

GTTCTAAGAGTGGATTCAACGAAGA

TTACAATTTCACCCCTGCTTAGTG

198

0.5435

4.0000

0.6027

0.5376

I5–6

25,186,057

GACTTTGACACCATTCGAGTTCAG

GTGTTTACCCCTTCTCACATAGGT

129

0.9348

4.0000

0.1243

0.1209

I5–9

40,002,310

TCATACTACTCTCCTGACCTCTCC

AATTGTGATGTTTTCTTGGAGGGC

287

0.7870

4.0000

0.3609

0.3362

I6–2

5,003,421

GGATAAATCTCCGAAATGCACTCT

GACAAGGTGATTTTGAAGAGTGGG

196

0.9217

3.0000

0.1470

0.1412

I6–6

25,003,694

TGGGCGAACTCAAGGTCAATATTA

CCTCTAGGCCTTCTCAGCTTAATT

157

0.7739

4.0000

0.3774

0.3487

I6–7

30,129,906

GTCTACAACATCTCCTCCACTCTC

ATTAAAATAGCCGCACGAAGAG

296

0.7000

4.0000

0.4429

0.3770

I6–8

35,088,175

TTTTGCTACTGGGAATTAGGCGAA

CAGAGGAGTCCAAGGAAGAAGAAA

280

0.4478

3.0000

0.6379

0.5629

I7–4

15,048,834

AAAATCCCAACCACACCGACC

CCACCACATCAAACCATTCAGATT

272

0.5652

3.0000

0.5255

0.4259

I8–2

5,036,397

AGCTCAATCTGCCCTTAGTTCTAC

GTTCATGTTCTCTTCCTCTCCTGT

224

0.4957

3.0000

0.5605

0.4634

I8–4

15,041,707

TACTGCAGGATATGTGTAAAGCGT

CACAATATGGGAGGAACAACAAGT

286

0.7652

5.0000

0.3950

0.3721