Fig. 4

Phylogenetic tree of limber pine NLR family constructed using maximum likelihood (ML) method based on alignment of NB-ARC sequences. Arabidopsis and rice sequences that were shown as the top-hits in BLASTp as queried by limber pine sequences were included and labelled with UniProtKB accession numbers. A total of 158 limber pine NB-ARC sequences with a minimum length of 150 amino acids were clustered with 41 Arabidopsis and 27 rice NB-ARC sequences. The phylogenetic branches or clusters with sequences exclusively from limber pine, Arabidopsis, and rice are indicated in black, blue, and red, respectively. The phylogenetic clusters containing sequences from both Arabidopsis and rice are shown in green. Most cluster are collapsed while the cluster with M117450 (in red) as Cr4 candidate is expended. Numbers near the nodes represent ML bootstrap values (> 20%)