Fig. 1
From: Reverse complementary matches simultaneously promote both back-splicing and exon-skipping
Large scale neuron isolation from C. elegans for circRNA detection. a. Workflow of neuron isolation and circRNA detection by RNA-sEq. b. Gating strategy for FACS: Forward scatter width (FSC-W) was plotted against forward scatter height (FSC-H) to select singlet cells (88.8 %), which were then used for the selection of GFP-positive and PI-negative cells (14.6 %) for sorting. c. Confocal images of sorted GFP-positive neurons. Scale bar: 50 μm. d. ddPCR results showing the relative levels of two genes (myo-3 and unc-64) in the sort group compared with those in the whole group. Error bars stand for standard deviations of three biological replicates. P values are ratio paired t-test