Fig. 7

Phylogeny of SPRYSEC effector genes of both Gr-Line19 (green) and Gr-Line22 (dark red). Only SPRY proteins with a signal peptide for secretion are included A multiple sequence alignment was made using MUSCLE on the coding sequence. A phylogenetic tree was made using RAxML using a GTRGAMMA model, validated by 100 bootstrap replicates. Bootstrap values < 50 % are indicated by a “-”. Closest homologs to the functionally described SPRYSEC-4, SPRYSEC-5, SPRYSEC-8, SPRYSEC-15, and SPRYSEC-19 [10] are shown. Clusters of SPRYSEC variants are boxed (A- E). Boxed clusters (Roman I – III) highlight asymmetric representations of variants among the two G. rostochiensis lines. Dashed lines are used in case there was uncertainty about the location of the 5’end of a given effector family variant