Table 1 Summary of SMRT-Seq dataset

Subreads base (G)

31.04

Subreads number

17,422,835

Average subreads length

1,782

Subreads N50

2,583

CCS

462,629

5’-primer

436,445

3’-primer

436,247

Poly-A

415,941

Full length

391,501

FLNC

385,367

Average FLNC read length

2,733

Polished consensus reads

220,474

Average consensus reads length

2,906

Polished N50

3,867

Total reads

220,474

Mapped polished consensus reads

185,869(84.3 %)

High-quality isoforms number

79,148

Isoform mean length

2,612

Isoform N50

3,261

Isoforms of known genes

1,999(2.5 %)

Novel isoform of known genes

53,448(67.5 %)

Isoforms of novel genes

23,701(29.9 %)

New genes number

18,827