Table 2 Artemia sampling for RNAseq, including sampling time after treatment start and ARC cyst bank ID
Treatment | Life cycle stage | Sampling time (min) | Biological replicates per sampling time | Cyst ID | Species | Strain |
|---|---|---|---|---|---|---|
Anoxia | cysts | 60 | 3 | ARC1767 | A. franciscana | SFB |
Hydration, normal oxygen | cysts | 60 | 3 | ARC1767 | A. franciscana | SFB |
High salinity (200 g/L) | instar I/II nauplii | 15, 30, 45, 60 | 3 | ARC1767 | A. franciscana | SFB |
Low salinity (30 g/L) | instar I/II nauplii | 15, 30, 45, 60 | 3 | ARC1767 | A. franciscana | SFB |