Fig. 3

Functions of genes expressed in the mouse seminal vesicle. A-C Ingenuity Pathway Analysis was used to annotate the core seminal vesicle transcriptome based on canonical signalling pathways, biological and disease functions, and upstream regulators of the expressed genes that were highly (p ≤ 0.05) predicted to be involved in mouse seminal vesicle function. Heat maps depict the top A 20 canonical signalling pathways based on –log10 p values, B 10 biological and disease functions based on number of target molecules in dataset, and C 20 upstream regulators based on –log10 p values. D A subset of ten genes from the mouse seminal vesicle transcriptome were selected for conventional PCR confirmation (n = 4). Representative agarose gel electrophoresis images of Thymoma viral proto-oncogene 1 (Akt), Caveolin 1 (Cav1), Cluster of differentiation (Cd)9 antigen (Cd9), CD47 antigen (Cd47), CD81 antigen (Cd81), Heat shock protein 90, alpha (cytosolic), class A member 1 (Hsp90aa1), Nectin cell adhesion molecule 2 (Nectin2), Milk fat globule-EGF factor 8 protein (Mfge8), Macrophage migration inhibitory factor (Mif) and Rapamycin-insensitive companion of mTOR (Rictor) detection with equivalent reverse transcription negative controls. Gel images are presented as cropped images for clarity and conciseness with corresponding full length gel images provided in Additional file 2, Figure A2