Redox proteomic study of Bacillus cereus thiol proteome during fermentative anaerobic growth

Hamitouche, Fella; Gaillard, Jean-Charles; Schmitt, Philippe; Armengaud, Jean; Duport, Catherine; Dedieu, Luc

doi:10.1186/s12864-021-07962-y

BMC Genomics

Table 2 Cys-proteins harboring specific cysteines detected as RedCys or as OxCys

From: Redox proteomic study of Bacillus cereus thiol proteome during fermentative anaerobic growth

	Cys position	Protein	Description	Log₂(FC)
	Cys position	Protein	Description	EEP/LEP	MEP/LEP
Cys proteins in EEP,MEP,LEP
Nucleotide metabolism
Q81IP8	381	PurD	Phosphoribosylamine-glycine ligase	NS	NS
Q81JC8	446	GuaB	Inosine-5′-monophosphate dehydrogenase	NS	NS
Defense mechanisms
Q81IE6	123	TerD	Tellurium resistance protein	NS	NS
Translation
Q812N7	170	PheT_1	Phenylalanine-tRNA ligase beta subunit	NS	NS
Cys proteins in EEP,MEP
Cofactors and vitamins metabolism
Q81J74	60		7,8-dihydroneopterin aldolase	NS	NS
Translation
Q819R4	124	IleS1	Isoleucine-tRNA ligase 1	NS	NS
Q814Q8	153	ArgRS	Arginine-tRNA ligase	NS	NS
Amino acid metabolism
Q814Q2	159	SpeB	Agmatinase	NS	NS
Cys proteins in EEP,LEP
Defense mechanisms
Q81GN6	458	Kat	Catalase	−2,46	−1,93
Cys proteins in MEP,LEP
Nucleotide metabolism
Q817X3	64	Apt	Adenine phosphoribosyltransferase	NS	NS
Q81IQ3	464	PurL	Phosphoribosylformylglycinamidine synthase subunit	NS	NS
Carbohydrate metabolism
Q818T4	111		2-oxoisovalerate dehydrogenase beta subunit	NS	NS
Q81IA3	123		Formate acetyltransferase	NS	NS
Cofactors and vitamins metabolism
Q818X5	92	RibH	6,7-dimethyl-8-ribityllumazine synthase	NS	NS
Q818R9	405	Dxs	1-deoxy-D-xylulose-5-phosphate synthase	NS	NS
Amino acid metabolism
Q817H8	189		Deblocking aminopeptidase	NS	NS
Lipid and Fatty acid metabolism
Q81IN1	38		DAGKc domain-containing protein	NS	NS
Pathogenesis
Q81I63	184		Microbial collagenase	NS	NS
Q81JC7	266		D-alanyl-D-alanine carboxypeptidase	NS	NS
Defense mechanisms
Q81GN6	357	Kat	Catalase	−2,46	−1,93
Transcription
Q81J66	312	MecB	Negative regulator of genetic competence	NS	NS
Cys proteins in EEP
Nucleotide metabolism
Q81IQ6	169	PurC	Phosphoribosylaminoimidazole-succinocarboxamide synthase	NS	NS
Q819S3	1034	CarB	Carbamoyl-phosphate synthase large chain	NS	NS
Q819S0	264	PyrB	Aspartate carbamoyltransferase	NS	NS
Q81FV5	38	DeoD	Purine nucleoside phosphorylase	NS	NS
Translation
Q81IN3	185	GatA	Glutamyl-tRNA (Gln) amidotransferase subunit A	NS	NS
Q814C5	258	FusA	Elongation factor G	NS	NS
Transcription
Q81J47	892	RpoC	DNA-directed RNA polymerase subunit beta	NS	NS
Amino acid metabolism
Q816W9	315		Deblocking aminopeptidase	NS	NS
Defense mechanisms
Q81IE5	110	TerD	Tellurium resistance protein	NS	NS
Function unknown
Q81IG4	65		Thiamine_BP domain-containing protein	NS	NS
Carbohydrate metabolism
Q81DX6	97	AdhA	Alcohol dehydrogenase	NS	NS
Cys proteins in MEP
Nucleotide metabolism
Q81J22	153	Adk	Adenylate kinase	NS	NS
Q81IS3	250	GuaA	GMP synthase	NS	NS
Q81IQ3	527	PurL	Phosphoribosyl formylglycinamidine synthase subunit	NS	NS
Translation
Q814G7	15	RpsR	30S ribosomal protein S18	NS	NS
Q81J29	27	RpsZ	30S ribosomal protein S14 type Z	NS	NS
Q814C4	82	Tuf	Elongation factor Tu	NS	NS
Transcription
Q81J47	818	RpoC	DNA-directed RNA polymerase subunit beta	NS	NS
Q81CE5	158	PpaC	Probable manganese-dependent inorganic pyrophosphatase	NS	NS
Amino acid metabolism
Q81GG4	115	TrpA	Tryptophan synthase alpha chain	−3.08	−2.14
Carbohydrate metabolism
Q818P6	194		Phosphoglycolate phosphatase	NS	NS
Q81DX6	91/255	AdhA	Alcohol dehydrogenase	NS	NS
Cofactors and vitamins metabolism
Q818X3	115	Biob	Biotin synthase	NS	NS
Protein folding
Q819C5	73		Peptidyl-prolyl cis-trans isomerase	NS	NS
Cys proteins in LEP
Amino acid metabolism
Q81GG5	378	TrpB	Tryptophan synthase beta chain	−2,24	−2,26
Q81GH0	168/65	TrpE	Anthranilate synthase component 1	−2,17	−2,17
Q81GG7	108	TrpC	Indole-3-glycerol phosphate synthase	−2,55	−2,26
Q81GG9	62	PabA (TrpGD)	Anthranilate synthase component II	−1,93	−1,39
Q814M2	364		O-acetylhomoserine sulfhydrylase	NS	NS
Carbohydrate metabolism
Q819M9	262		Pyruvate carboxylase	NS	NS
Q81FZ7	124		Glycerate dehydrogenase	NS	NS
Q81A22	50	PorB	Pyruvate synthase subunit	NS	NS
Q81IA2	12	PflA	Pyruvate formate lyase activating enzyme	NS	NS
Q817F3	283	PfkA	ATP-dependent 6-phosphofructokinase	NS	NS
Q819D7	365	Ptsɪ	Phosphoenolpyruvate-protein phosphotransferase	NS	NS
Cofactors and vitamins metabolism
Q81JC6	128/130	PdxS	Pyridoxal 5′-phosphate synthase lyase	NS	NS
Q816H9	71	MenB	1,4-dihydroxy-2-naphthoyl-CoA synthase	NS	NS
Q818W9	69	BioD	ATP-dependent dethiobiotin synthetase	NS	NS
Transcription
Q81J18	265	RpoA	DNA-directed RNA polymerase subunit alpha	NS	NS
Translation
Q814C4	138	Tuf	Elongation factor Tu	NS	NS
Nucleotide metabolism
Q81JC8	327	GuaB	Inosine-5′-monophosphate dehydrogenase	NS	NS
Defense mechanisms
Q81IK9	166	AhpC	Alkyl hydroperoxide reductase C	NS	NS
Lipid and Fatty acid metabolism
Q814Y7	91	FabZ	3-hydroxyacyl-[acyl-carrier-protein] dehydratase	NS	NS
Q816B6	46/49	NfuA (YutI)	NifU protein	−1.9	−1,68
Q816D2	117		HESB protein	NS	NS
Secondary metabolites biosynthesis, transport and catabolism
Q81DQ3	14	DhbC	Isochorismate synthase	−2.71	−3.06
Q81DQ1	67	DhbB	Isochorismatase	−2.55	−2.52
Energy production and conversion
Q814U7	135		Nitrilotriacetate monooxygenase component B	NS	NS

Protein identifiers are underlined for proteins with differences in abundance; Cysteine positions are underlined for Cys-peptides detected with a higher proportion of RedCys at LEP; FC fold change; NS not significant

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ISSN: 1471-2164

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