Fig. 2

Differential expression of microribonucleic acids (miRNAs) in diaphragm tissue after mechanical ventilation. A Statistics of DE miRNAs. B Volcano plot of miRNA expression levels. The red dots indicate upregulated miRNAs with significantly differential expression, the green dots indicate downregulated miRNAs with significantly differential expression, and the grey dots indicate miRNAs without differential expression. The X-axis shows the log₂ fold change values; the Y-axis shows the -log₁₀ p values. C Heat map of DE miRNAs. Each row represents one probe set; each column represents one single sample. The dendrogram at the top reveals sample clustering; the dendrogram to the left reveals gene clustering